Effect of inactivated Tsukamurella inchonensis on post vaccination serum titer ‎against infectious bursal disease in broiler chicks

The present study was designed to investigate the effect of inactivated Tsukamurella inchonensis (T. inchonensis) on the immune response against infectious bursal disease (IBD) vaccination in broiler chicks. Ross broiler chicks in the cage (n= 180; one day old) were randomly assigned to five groups (36 birds in each group). Experimental diets were prepared by adding 106 cells per bird of inactivated T .inchonensis into the commercial basal diet. For group the treatment was continuously dosed during 24 h from day 1 to day 13; and for group 2 during 24 h on days 1 to 5; 8; 9, 12 and 13. Group 3 was received 106 bacteria as subcutaneous injection on days 1, 6, and 12. Groups 4 and 5 weren't received T. inchonensis during the experiment period. Blood was collected on days 1, 14, 28, and 42 from the wing vein of 12 birds, randomly per treatment. Serum IBD antibody titer were studied by ELISA, which measured by double-antibody sandwich ELISA using commercial kits. T. inchonensis treatments, irrespective of the routes of delivery, increased the antibody titers to IBD vaccines, especially when broiler chickens treated with pulse dosed in the feed (p < 0.05). T. inchonensis augmented the effects of IBD vaccination in strengthening subsequent anti-viral responses.