Investigation on population genetic structure of autumn and spring migratory Caspian lamprey (Caspiomyzon wagneri Kessler, 1870) in the Shiroud River by using microsatellite molecular technique

The genetic diversity of Caspian lamprey, (Caspiomyzon wagneri) was studied using  the microsatellite technique in Shiroud River. 67 caudal and dorsal fin samples were collected from spawned Caspiomyzon wagneri caught from Shiroud River in autumn 2015 and spring 2016 seasons. Genomic DNA was extracted using ammonium acetate method and polymerase chain reactions (PCR) were conducted using 10 paired microsatellite primers. The quantity and quality of DNA were assessed using spectrophotometry and a horizontal electrophoresis in 1% agarose gel methods and were stained using silver nitrate method. The results showed that it was produced six polymorphic and four monomorphic loci. The average real and effective number of alleles in autumn migrant were 2.66 and 1.88 and 3.16 and 2.06 in spring migrant. The mean observed and expected heterozygosity in autumn migrant were calculated 0.239±0.101 and 0.363±0.116 and 0.374±0/110 and 0/434±0/093 in spring migrant, respectively. More loci were out of Hardy-Weinberg equilibrium (p < 0.05). Based on the analysis of molecular variance, Fst and Rst were 0.153 and 0.200 that showed significant differences (p < 0.01). This study gave evidence of the presence of two separated populations in studied river that should be considered in restructuring reserves.