Role of Cetrorelix in preservation of morphological and ultrastructural changes in testicular epithelium germinal in Cyclophosphamide-induced toxicity in mouse

Anticancer drug that used in treatment of cancer has negative effect on some organs that rapid division such as testis tissue. Gonadotropin releasing hormone (GnRH) antagonist may has a preventive effect on spermatogenic defect produced by anticancer drug such as cyclophosphamide. Therefore, the aim of the present study is to investigate the preventive effect of GnRH antagonist (Cetrorelix) on cyclophosphamide-induced defect. In the present study, thirty adult male mice aging 6-8 weeks were divided into three groups; control group, Experimental 1 (50mg/kg BW cetrorelix with cyclophosphamide, intraperiteonally) and Experimental 2 (0.25 mg/kg, intraperiteonally). The mice were sacrificed 35 days after the last injection of cetrorelix and testes speciemen were isolated for histomorphological and ultrastructure studies. Histomorphometric studies of the seminiferous tubules in Experimental 1 showed significantly decreased in the number of sertoli cells and the thickness of germinal epithelium (p < 0.05). Ultrastrctural study revealed that several intercellular spaces appear between sertoli cells and spermatogenic cells, and also there were a lot of degenerated mitochondria in the sertoli cells. In Experimental 2, condition was similar to control group to someextand. These results demonstrated that cetrorelix can protect germinal epithelium of testis to someextand against side effects of cyclophosphamide.