Effect of Spinal Nerve Ligation (SNL) on the expression of TRAF6 and MuRF1 genes in soleus mucsle of Wistar rats after HIT training

The purpose of the present study was to investigate the effect of SNL on the expression of TRAF6 muRF1 genes in the Soleus muscle of Wistar rats after HIT traning. For this purpose, 24 male rats were divided into 2 groups of control (C) (n=12) and training (HIT) (n=12). After one-week familiarization, training group participate in four HIT training. Then, they were randomly assigned to HIT (n=6) and HIT-SNL (n=6) groups. At the same time, the control group was divided into two groups: control (C) (n=6) and spinal cord nerve (C-SNL) (n=6). 4 weeks after the SNL, wistar rats were sacrificed and soleuse muscle exetracted.Then Gene expressions of MuRF1 and TRAF6 measured Real time PCR technique. The results showed that inactivity by SNL has a significant effect on the expression of TRAF6 and MuRF1 genes (P=0.0001 and P=0.0001, respectively). Also, performed before SNL reduced the expression of TRAF6 and MuRF1 (P=0.003 and P=0.0001 respectively). In addition, the findings indicated that SNL had a significant effect on the weight of Soleus muscle mass/ tibia length (P=0.01). On the other hand, HIT training before SNL significantly increased weight of Soleus muscle mass/ tibia length (P = 0.03). Therefore, the decrease in activity by SNL is associated with increased in expression of TRAF6 and MuRF1 genes, which may also be involved in muscle mass changes. Regarding the fact that performing HIT reduces the expression of these genes in soleus muscle, these training can be used to maintain obtimal muscle mass before physical activity.