Physiological and biochemical changes and calcium-dependent protein kinase expression in canola (Brassica napus L.) under salinity stress

Salinity stress is one of the most important of abiotic stress that affects the yield of oilseed rape. In order to study some physiological and biochemical changes and BnaCDPK14 transcript expression in rapeseed (Brassica napus L.), two tolerant cultivars (Slm046 and Zarfam) and two susceptible cultivars (Okapi and Sarigol) were planted in a growth chamber and were irrigated by water including 100 and 200 mM NaCl and normal water. Relative water content, electrolyte leakage, antioxidant enzyme guaiacol peroxidase (GPOX), antioxidant enzyme catalase (CAT) and the expression of calcium-dependent protein kinase 14 (BnaCDPK14) were measured. The results indicated the relative water content and electrolyte leakage (200 mM NaCl) decreased and increased under stress respectively. The antioxidant enzyme guaiacol peroxidase (GPOX), catalase (CAT) and BnaCDPK14 increased by salinity stress, tolerant cultivars showing more increase. Negative correlation was observed between the relative water content of leaves and electrolyte leakage. There was a high positive correlation between the guaiacol peroxidase and catalase contents and the expression of BnaCDPK14, indicating that by increasing the reactive oxygen species under stress, the plant enzymic antioxidant system helps the plant to cope with it. Salinity stress is one of the most important of abiotic stress that affects the yield of oilseed rape. In order to study some physiological and biochemical changes and BnaCDPK14 transcript expression in rapeseed (Brassica napus L.), two tolerant cultivars (Slm046 and Zarfam) and two susceptible cultivars (Okapi and Sarigol) were planted in a growth chamber and were irrigated by water including 100 and 200 mM NaCl and normal water. Relative water content, electrolyte leakage, antioxidant enzyme guaiacol peroxidase (GPOX), antioxidant enzyme catalase (CAT) and the expression of calcium-dependent protein kinase 14 (BnaCDPK14) were measured. The results indicated the relative water content and electrolyte leakage (200 mM NaCl) decreased and increased under stress respectively. The antioxidant enzyme guaiacol peroxidase (GPOX), catalase (CAT) and BnaCDPK14 increased by salinity stress, tolerant cultivars showing more increase. Negative correlation was observed between the relative water content of leaves and electrolyte leakage. There was a high positive correlation between the guaiacol peroxidase and catalase contents and the expression of BnaCDPK14, indicating that by increasing the reactive oxygen species under stress, the plant enzymic antioxidant system helps the plant to cope with it. Salinity stress is one of the most important of abiotic stress that affects the yield of oilseed rape. In order to study some physiological and biochemical changes and BnaCDPK14 transcript expression in rapeseed (Brassica napus L.), two tolerant cultivars (Slm046 and Zarfam) and two susceptible cultivars (Okapi and Sarigol) were planted in a growth chamber and were irrigated by water including 100 and 200 mM NaCl and normal water. Relative water content, electrolyte leakage, antioxidant enzyme guaiacol peroxidase (GPOX), antioxidant enzyme catalase (CAT) and the expression of calcium-dependent protein kinase 14 (BnaCDPK14) were measured. The results indicated the relative water content and electrolyte leakage (200 mM NaCl) decreased and increased under stress respectively. The antioxidant enzyme guaiacol peroxidase (GPOX), catalase (CAT) and BnaCDPK14 increased by salinity stress, tolerant cultivars showing more increase. Negative correlation was observed between the relative water content of leaves and electrolyte leakage. There was a high positive correlation between the guaiacol peroxidase and catalase contents and the expression of BnaCDPK14, indicating that by increasing the reactive oxygen species under stress, the plant enzymic antioxidant system helps the plant to cope with it.