Effect of different levels of canola meal and protease enzyme on performance, egg quality traits and nutrient digestibility in laying hens

This experiment was conducted to consider different levels of canola meal (CM) and protease enzyme on performance, egg quality, nutrient digestibility, intestinal aminopeptidase activity, and relative weight of digestive organs of laying hens from 28 to 36 weeks of age. In total, 180 Hy-Line W-36 laying hens were allocated to six treatments, five replicates, and six laying hens in each replication in a factorial arrangement of 3×2 by a completely randomized design with three levels of CM (0, 7.29, and 14.59%) and two levels of protease enzyme (0 and 200 g/ton). For the entire experimental period, the productive performance of laying hens and egg quality were not affected by CM levels or protease enzyme (P>0.05). As well as, there were no significant effect of CM levels and protease enzyme on AMEn, protein, and organic matter digestibility of the diet (P>0.05). The aminopeptidase activity of the duodenum (P<0.01) and jejunum (P=0.0511) was significantly increased by 14.59% CM supplemented with protease enzyme (10.61 and 11.94 Unit/ mg of tissue, respectively). Duodenal aminopeptidase activity increased by protease enzyme supplementation (7.74 vs. 6.69 Unit/ mg of tissue) (P=0.0103). The results showed that CM can be included in laying hen diets up to 14.59% with protease enzyme without any adverse effect on productive performance, egg quality traits, and nutrient digestibility.
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