Assessment of pathogens microorganisms of periapical abscess in primary teeth

Dental abscess is considered as one of the most common complications of untreated caries in children. Microorganisms are known as major causes for the pulp and periradicular pathosis. Black – pigmented, gram – negative anaerobes, belonging to species of porphyromonas and prevotella (bacteroides) play an important role in causing clinical signs and symptoms related to pulp and periradicular disease. The aim of this investigation was to assess the presence of two main pathogenic microorganisms named porphyromonas gingivalis and prevotella melaninogenica in abscessed primary teeth. Methods & Materials: Forty children aged 4-10 years were selected with no systemic disease and no antibiotic consumption during the last two weeks of sampling. Selected teeth were presenting swelling and pain at observation stage as signs of acute abscess. The diagnostic technique of porphyromonas gingivalis and prevotella melaningenica was the use of DNA formulation reading by means of PCR technique. Results showed that of the 40 samples, 35(%87.5) were positive for porphyromonas gingivalis, 34(%85) were positive for prevotella melaninogenica. However, it seems that more work is required for more precise antibiotic therapy of dental abscess in children. The presence of two well known species of microorganisms in periapical abscess of primary teeth was confirmed.