A Comparison of the Effect of Two Different Orders of Combined Training on Inflammatory and Muscle Atrophy Biomarkers in Women with Type 2 Diabetes

The aim of this study was to compare the effect of two concurrent training (resistance training + high-intensity interval training (HIIT)) with different orders on inflammation caused by diabetes, inflammation-induced muscle atrophy, insulin resistance, and body composition in women with type 2 diabetes. 24 nonathletic 45-65 year-old women with type 2 diabetes voluntarily participated in this study. They were divided into three groups: control group, type I, and type II exercise. Both groups of exercises performed concurrent training for 8 weeks. The first group had both exercises on the same day in the form of resistance-HIIT and the second group had the same volume of exercise on separated days. Cardiovascular evaluation and body composition analysis were measured before and after the training period. Blood samples were collected 24 hours before the first session and 48 hours after the last session of concurrent training to measure glucose, insulin, glycosylated hemoglobin, IGF1, and IL1β. Analysis of covariance (ANCOVA) was used for comparison between groups and the results of post-test analysis of covariance showed a significant decrease in insulin resistance in both training groups compared to the control group. IL1β decreased significantly in both training groups compared to the control group and there was no significant difference between the two orders. The increase in IGF1 and fat-free mass were significant in both training groups compared to the control group and a significant difference was observed between type I and type II exercise (p < /em><0.05). The results of this study showed that concurrent training with two different orders can be an important factor in decreasing insulin resistance and inflammation caused by diabetes and increasing IGF1 growth factor while exercise on separated days had more beneficial effects on boosting muscle growth.