Protective Effects of Gamma Oryzanol on Renal and Brain Damage Following Experimental Renal Ischemia-reperfusion Injury among Rats

Renal ischemia/reperfusion injury is one of the major causes of acute renal failure. This study aimed to investigate the effects of gamma oryzanol administrated by gavage and intraperitoneal methods on the antioxidant function and status of the kidneys and brain tissue after the induction of ischemia-reperfusion injury in the kidneys of rats.

The statistical population of this study consisted of 24 male rats divided into four 6-member groups, including the sham group without ischemia, control group with ischemia-reperfusion induction, ischemia-reperfusion group receiving treatment with 100 mg/body weight intraperitoneally, and ischemia-reperfusion group administered with a dose of 100 mg/body weight by gavage method. Gamma oryzanol was administered 1 h followed by the onset of left renal ischemia for 30 min. After 6 h of reperfusion, blood creatinine and urea and antioxidant function of the kidneys and brain tissue were measured.

The results showed that the administration of gamma oryzanol by both methods of intraperitoneal injection and gavage caused a significant reduction in creatinine and blood urea levels, compared to the control group (P<0.05). Moreover, the amounts of glutathione peroxidase, glutathione reductase, superoxide dismutase, catalase, and total oxidant capacity of kidney and brain tissue were significantly increased in the groups receiving treatment with gamma oryzanol; however, malondialdehyde level was significantly reduced in the same group, compared to the control group (P<0.05).

Based on the results, gamma oryzanol improved renal function and reduced the negative severity of ischemia-reperfusion injury in renal and brain tissues. It was also found that the gavage method performed better than the intraperitoneal injection method.