Seroepidemiological Analysis of Leptospiral infection using MAT in Stray Dogs in Alborz, Iran

Leptospirosis is a zoonotic disease with global importance, and the animals are the source of transmission of this disease through shedding in their urine. Accordingly, it is essential to conduct epidemiological studies of leptospirosis in order to diagnose this disease in dogs and reduce the risk of transmission to humans. This study aimed to perform a seroepidemiological analysis of Leptospiral infection in stray dogs in Alborz, Iran, using the Microscopic Agglutination Test (MAT). In total, 110 blood samples were collected from stray dogs to detect the antibodies against leptospira interrogans serovarsby the MAT.  The prevalence rate of positive MAT tests in stray dogs was estimated at 21.84%. The following protocol confirmed that the most common titers were 1:200 (50%) and 1:400 (25%). In addition, the most prevalent Leptospira serovars were L. Canicola (33.33%), and the lowest belonged to L. Pomona (4.1%). Moreover, no significant difference was observed between the age and gender of the dogs regarding their MAT titer (P>0.05). The results also showed a high prevalence of leptospirosis in stray dogs of Koohsar in Alborz province, Iran. Since Leptospirosis is a zoonosis disease, it should be studied continuously in humans and animals, especially dogs.