Investigation the effect of media and indolebuteric acid hormones (IBA) on rooting of grape cuttings by inverted culture

In order to investigate the effect of media and Auxin plant growth regulator on rooting of commercial grape cuttings, an experiment was conducted in a factorial arrangement based on a complete randomized block design with three replications on ‘White Seedless’ grape cuttings. Various types of media (sand, sawdust, agronomy, combined soil) and different concentrations of indole butyric acid (0, 2 and 4 g /l) were used as a treatments. After applying treatments, some traits such as callus start time, callus percentage, time of onset of misplaced roots, root length, root number and percentage of cuttings were measured. All measured traits significantly affected by various media (except percentage of cuttings) and different concentrations of IBA (p < 0.01) and their interactions. The results of experiment revealed that the interaction effect of sawdust and 4 g/l IBA significantly (p < 0.01) decreased times for callus and adventitious root inductions and also produced the highest percentage of callus, root number and length, and rooting ability. The highest percentage of rooting was obtained in cuttings which treated by combined soil and 2 g/l IBA. However, it’s concluded that IBA by decreasing the callus induction time and increasing cell division and root number was played a significant role in rooting of cuttings. In fact, in sand and sawdust media with consideration the production of higher root densities, the percentage of rooting was reduced due to lack of food.