First Report of Some Fungal Species on the Potato Golden Cyst Nematode, Globodera rostochiensis in Iran

Potato golden cyst nematode(Globodera rostochiensis) is the most destructive plant-parasitic nematode of thise crop and surching for variuse control ways is very essential.. Because of the chitin is a dominant composition in middle layer of the eggshell in nematodes, using of the chitinases by chitin-degrading enzymes in a wide range of the fungi is a good strategy for biological control of the potato golden cyst nematode. In this research 154 fungal isolates were recovered from infected eggs of the potato golden cyst nematode, G. rostochiensis. These isolates were identified based on morphological and molecular features including internal transcribed spacer regions (ITS1, ITS2 and 5.8S gene) of ribosomal DNA.. The ITS1, ITS2 and 5.8S sequences were obtained by sequencing both strands in opposite directions using the PCR amplification primers, ITS1 and ITS4 in genomics resource laboratory at Massachusetts University, USA. In this study, according to morphological features and sequencing of ITS regions of ribosomal DNA, these 154 isolates were classified in 13 genera, Alternaria, Aspergillus, Beauveria, Candida, Chaetomium, Cylindrocarpon, Fusarium, Trichocladium, Lecanicillium, Plectosphaerella, Purpureocillium, Trichoderma andUlocladium. The five fungal species Candida parapsilosis, Cylindrocarpon olidum, Trichocladium griseum, Plectosphaerella cucumerina and Ulocladium dauciare reported for the first timefrom G. rostochiensis in Iran. As a producer of various enzymes, the filamentous fungi has been suggested as an important means of biological control for G. rostochiensis.