Effects of Nicotine on Expression of CXCR4, CD49d, CD11a, CD18, CD62L Markers on Human Cord Blood CD34+ Cells

Smoking can have many adverse effects on human health and nicotine as a main gradient in cigarette smoke is the best topic of related research. The goal of this study was exploring the effects of nicotine on expression of CXCR4 as a chemokine receptor and adhesion molecules on hematopoietic stem/progenitor cells. For this purpose after isolation of mononuclear cells from umbilical cord blood (UCB) they were cultured for 24 hours in three doses of 10nM, 1μM and 100μM nicotine. Then, the expression of CXCR4, CD49d, CD11a, CD18 and CD62L on HSPCs (CD34+ cells) was determined by flow cytometry. Nicotine decreased cellular expression of CXCR4. But the expression of other factors unless CD62L were increased. It can be Concluded in in vitro condition nicotine alters expression pattern of CXCR4 and CD11a, CD18, CD49d.