Investigation of Performance and Comparison of Cytochrome P450 Isoforms in Broiler Chickens Liver Fed Silver Nanoparticles Coated on Clinoptilolite

In order to evaluate the effect of silver nanoparticles coated by clinoptilolite on cytochrome P450 gene expression in broiler chicken’s liver an experiment consisting of (1) control diet, (2) clinoptilolite, (3) clinoptilolite coated with silver nanoparticles, (4) organic acids and (5) clinoptilolite coated with silver nanoparticles and organic acids in normal and heat stress conditions was designed. Liver biopsy was obtained on 21 and 42 days of experiment. Results of this study demonstrated there were no interaction between experimental treatments and heat stress conditions in chickens. As well as the expression levels of  both isoforms of CYP450 gene  in clinoptilolite treatment on day 21 and silver nanoparticles coated on clinoptilolite with organic acid treatment on day 42 of experiment in normal conditions, were significantly increased in comparison with control treatment (C) in chicken liver tissue (P<0.05). Level of CYP2C18 on  day 42 of experiment in heat stress condition in silver nanoparticles coated on clinoptilolite treatment was significantly increased in comparison with control (C) treatment (P<0.05). Whereas, the level of CYP2C23b gene expression on day 42 of experiment was not significantly different with control treatments (P>0.05) in heat stress conditions. In conclusion, results showed that the expression level of CYP2C18 isoform in liver tissue of broiler chickens was higher in comparison with CYP2C23b isoform.  The liver, as a metabolism organism in the body, identified clinoptilolite and silver nanoparticles as inorganic, chemical and exogenous substances and increased the expression level of cytochrome P450 gene acts as a biomarker for the existence of exogenous and inorganic substances in the body.