Regeneration of Plantlets from Fragmented Embryo Explant of Parsi Zira (Bunium persicum Boiss.)

Parsi zira (Bunium persicum Boiss.) that is also called mountainous or black zira, is one of the most important medicinal plants with high economic importance. There is a little information about in vitro culture of Parsi zira. Fragmented embryo was used as an explant in Bunium persicum regeneration. In this method, a great callus induction and regeneration occurred only on the same medium without any subculture. In this research, MS media containing different concentrations of plant growth regulators, NAA and 2,4-D alone or together with Kin, were used. The experiment was conducted using completely randomized design with 30 treatments and 10 replications per treatment. The treatment containing 0.1 mgl-1 NAA and 2 mgl-1 Kin was the best one for regeneration. The treatment containing 0.1 mgl-1 NAA and 0.5 mgl-1 Kin was the best one for somatic embryogenesis. Regeneration occurred in some treatments without kinetin, showing that kinetin is not essential for Bunium persicum regeneration