Effect of Different Concentrations of Benzyladenine (BA) and Naphthalene Acetic Acid (NAA) on Regeneration of Lilium ledebourii (Chelcheragh Lily) Using Bulblets Microscales

  Lilium ledebourii (Chelcheragh lily) is a unique species of Lilium genus that is native of north Iran. This study was conducted to investigate the bulblet regeneration of microscales of Chelcheragh lily in vitro conditions and to examine the effect of growth regulators benzyladenine (BA) and naphthalene acetic acid (NAA) in different concentrations. Solid MS medium with three concentrations 0, 0.01, and 0.1 mgl-1 of BA and NAA was used. The experimental design was a 3×3 factorial. Results showed that microscales had good regeneration potential of bulblet, especially when growth regulators used in suitable concentration. The highest number of bulblets and roots obtained in 0.01 mgl-1 BA and 0.1 mgl-1 NAA. Some of regenerated bulblets produced scale leaf directly by increasing the concentration. This character affects negatively bulblet regeneration of L. ledebourii. The results of the present study showed the possibility of micropropagation of L. ledebourii by microscales. Considering the lack of plant material and low vegetation of this plant in natural habitat, these results may find practical application for propagation of this rare plant.