Production of Doubled Haploid Lines of Wheat Using Detached Tillering Method in Cross Between Wheat and Maize, and Evaluation of some Agronomic Characters

In this study, chromosome elimination method was used to develop doubled haploid lines of wheat via crosses with maize. The plant materials were F1 seeds including Kavir/Zagros, Roshan (winter backcross)/ Zagros and Rsh2* 10120/Zagros along with three maize genotypes H1=KSC108, H3=KSC301 and H7=SC704 as pollin source. Two methods of haploid production in wheat involving A: conventional technique and B: detached-tiller culture were used and compared. The traits such as seed set percentage, embryo development and haploid seedling development were studied. As a result of this study, 75 lines of doubled haploid wheat were developed. It was also found that the second method (B) was better than the first method (A). In field, traits such as seed number per spike, 1000 grain weight, heading date and plant height were evaluated and finally 15 doubled haploid lines were selected.These lines are now evaluating in Advanced Regional Wheat Yield Trial.