Determination of HUWA-SAN TR-50 (Hydrogen peroxide+silver) LC50 in Rainbow trout (Oncorhynchus mykiss) eggs

Fungal infections are important in the destruction of freshwater fishes and their eggs in the wild and commercial fish farms. This study aimed to investigate the lethal concentration of HUWA-SAN TR50, which is among the new generation of ecological disinfectant compounds. For thispropouse, atotalof 4000 fertilized eggs of rainbow trout were used in 5 treatments and one control, and each group with 3 repetitions. During the experiment, the water temperature, DO, Ph and hardness were 11±0.5°C, 8.6±0.32 mg/L, 7.7±0.41 and 130 mg/l, respectively. During the experiment, the incubators were oxygenated by aeration pumps and mortality was recorded every 24 hours.  The results showed that the mortality percentage in treatments was significant different compared to the control one (p <0.05). Therefore, LC50 of this substance was calculated in 24 hours as 2278.12, in 48 hours as 1251.35, in 72 hours as 552.31 and in 96 hours 312.40 ppm. Hence the LC50 96 h concentration of HUWA-SAN TR50, is close to therapeutic dosage and it has a relatively more different than its toxic doses. Therefiren, this can be recommended in practical treatment in Rainbow trout hatcheries.