Bioinformatics design of a vaccine against Vibrio cholerae using the reverse approach

Vaccine design from the past to the present is based on classical methods, which are time consuming, costly and have some risks, but with the discovery of the genome and gene sequencing, the modern methods for vaccine design have been developed, including Referred to the reverse method of vaccine design (vaccine polytop). In this study we used NCBI (ORF Finder) to find the coding sequences, PSORTb server to locate the protein, Algpred server to check the allergenicity of the protein and LBtope - ABCpred - IEDB CBTOPE servers - Disco Tope 2.0 - IEDB (Ellipro) to predict the linear and spatial epitopes. The final epitope was selected and then we analyzed the polypepe structure by ExPasy server (ProtParam) and finally obtained the optimized nucleotide sequence with JCat server. Using this method, 6074 reading frameworks as well as 43 surface and secretory proteins were identified, from which, finally, 10 suitable epitopes that are able to provide immunity against a wide range of strains were selected as a vaccine candidate. It is hoped that this will facilitate the development of this vaccine.