Evaluation the Effect of Coronatine Pretreatment on Stress Zinc Oxide Synthesized by Olive on Soybean plant

Green synthesis by plant extract has achieved a growth in interest. In the present study, zinc oxide nanoparticles were synthesized by olive extract.Morphological and structural properties of the synthesized zinc oxide nanoparticles have been characterized using UV-Vis spectrophotometer, TEM analysis. Then, the effect of zinc oxide nanoparticles at concentrations of 0, 200 and 400 ppm with pretreatment coronatine (0 and 50 nM) on the parameters of hydrogen peroxide and malondialdehyde and catalase (CAT) enzymes, ascorbate peroxidase (ASP), superoxide dismutase (SOD), phenylalanine ammonia lyase (PAL) was investigated. Synthesized zinc oxide nanoparticles were confirmed by maximum absorption at wavelength of 360 nm. TEM image revealed that zinc oxide nanoparticles were spherical with average size 41 nm. Investigation of zinc oxide nanoparticles on soybean showed that at concentrations of 400 ppm zinc oxide nanoparticles, enzyme activity of ASP, PAL, SOD, hydrogen peroxide and malondialdehyde increased. The content of malondialdehyde at a concentration of 200 ppm of zinc oxide nanoparticles did not change significantly compared to the control, but the activity of the SOD enzyme increased significantly compared to the control. Pretreatment coronatine improved stress at 200 ppm zinc oxide nanoparticles and reduced the content of hydrogen peroxide and malondialdehyde, but coronatine increased this content at 400 ppm zin oxide nanoparticles.