Design and Manufacture of Modified Alginate Spray as a New Wound Dressing to Control Bleeding and Wound Healing

Alginate is one of the substances that is considered as a promising candidate for wound healing. The aim of this study was to design and manufacture a dressing spray based on alginate / calcium chloride / gelatin alginate to control bleeding and wound healing.

This experimental research was conducted in the research laboratory of Amirkabir University from December 2019 to January 2020. In this study, saline phosphate buffer solution was prepared first. Then, their sterilization process was performed by autoclave and MTT salt solution was prepared during a process. In order to prepare an alginate-based spray dressing, we adjusted the concentration of the solution so that it could escape from the spray pore. On the other hand, in order to obtain gel alginate with suitable strength in equal proportions of calcium chloride concentrations, aqueous solution of gelatin was prepared for copolymerization with alginate. Finally, the bonds in the proposed dressing were evaluated by FTIR. Also, the degree of degradability, water absorption, changes in weight and pH, physical properties and microstructure of the extrinsic biological response were investigated in the designed dressing. Scanning electron microscopy and infrared spectroscopy were used to examine the dressing structure resulting from the ratio of different amounts of materials involved.

The results showed that the amount of water absorption in the sample and their degradation rate were AGC3> AGC4> AGC2> AGC1. In the study of biocompatibility, it was found that despite better results in AGC3, the degree of non-toxicity of AGC2 group was higher and also showed better cell adhesion behavior. Therefore, according to the results, AGC2 sample was selected as the final sample.

The results revealed that increasing calcium chloride levels could lead to increased alginate hydrogel formation. Also, the presence of gelatin and calcium chloride in the constant concentration of alginate will reduce the formation time of the spray hydrogel until they create space barriers.