Investigation of proteome changes of susceptible and resistant cultivars of tomato leaves in response to pathogenic fungus Alternaria solani

Early blight is one of the major diseases of tomato that reduce the quality and quantity of the product. Regarding the molecular mechanisms involved in the host defense to the pathogen, little information is available to realize the mechanisms of defense response of tomato to pathogenic fungus Alternaria solani. In this research, the protein profile (proteome) of tomato leaves were investigated using two-dimensional electrophoresis. A total of 610 detection protein showed 29 different expression proteins in resistant and susceptible cultivars. These proteins mainly involved in stress and defense, energy and metabolism, photosynthesis, protein biosynthesis, and signal transduction. Proteins involved in stress and defense such as pathogencity related proteins (PR) and biosynthesis of phenolic metabolite were the most frequent. The most important defense mechanisms of the plant against A. solani were strengthening of the cell wall and antimicrobial phenolic compounds. The results of this study can be used to production of resistant varieties of tomato against A.solani.