Surface modification in microfluidic platform to miR-21 and miR-486 detection from lung cancer cell

Circulating miRNAs have also been proposed as novel biomarkers for early diagnosis and even prognosis of cancer. Due to the non-invasive access, in addition to before metastase early detection, the use of these molecules can reduced injuries of patients. In this study, the biosensor was designed to isolate circulating mi RNAs by microfluidic system. The design was based on the isolation of two miRNAs (miR-21 and miR-486), which have been described in papers as miRNAs with potential to diagnosis of lung cancer.In this system, miRNA isolation is performed using a capture probe (single-strand DNA), immobilized by GPTMS linker on PDMS surface. By attaching of microRNA to this probe, the second probe that was biotinilated DNA complement to upper portion of microRNA, attaches to it and creates a sandwich structure. Eventually, trapped microRNA between two probes was detected by FITC- streptavidin. miRNA has been visualized under IX81 Olympus florescent microscope.