Assessment of regeneration of Potato cultivars using in vitro culture under different hormonal compositions

Low frequency of transformation and, more importantly, the occurrence of soma clonal variation at very high rates have considered disadvantages of Potato In vitro culture. Two Phytohormones, auxins (NAA, 2, 4-D), and cytokines (BAP, ZR) with concentrations were used to develop an efficient regeneration protocol for three genotypes of Potato.

The explants cultured on MS-medium supplemented with BAP 1 mg.l-1; NAA 0.1 mg.l-1; BAP 2 mg.l-1; NAA 0.2 mg.l-1; BAP 3 mg.l-1; NAA 0.3 mg.l-1; ZR 1 mg.l-1; 2.4-D 0.1 mg.l-1; ZR 2 mg.l-1; 2.4-D 0.2 mg.l-1; ZR 3 mg.l-1; 2.4-D 0.3 mg.l-1.

The results showed that the highest percentage and number of root formation and shoot regeneration obtained in the medium included BAP 3 mg.l-1; NAA 0.3 mg.l-1 or ZR 3 mg.l-1; 2.4-D 0.3 mg.l-1 respectively. In this study, 0.3 mg.l-1 of both NAA and 2, 4-D were able to induce the most effective root regeneration.

The three potato genotypes were statistically different in their leaf regeneration efficiency. The Odyssey genotype showed a weak response to leaf regeneration.