The Effect of Resveratrol Antioxidant in Nano Liposome and NLC Forms in Rooster Semen Extender During Storage at 4˚C

The purpose of this study was to investgate resveratrol nano-protected antioxidant on rooster sperm. In this study, resveratrol was used in the forms of nano-protected (liposome and NLC) and plain in the semen extender. Immediately after the semen collection and for primary evaluations, for eliminating of individual effects the semen collected from 15 roosterswere pooled and added to the extender. Extending was done at 37 °C at a dilution ratio of 1:20. Different concentarions of resveratrol (20, 40 or 60 μM) were added to the extender in three forms of plain, NLC, and liposome. After diluting the semen, gradual cooling was carried out for 3 h at 4 °C. After the cooling process, the kinetic parameters (CASA), membrane integrity (HOST), viability, lipid peroxidation (MDA), abnormal sperm, were evaluated. The results at 24 and 48 hours at a temperature of 4°C indicate that the use of 40 μM resveratrol, resveratrol loaded in the liposome, and resveratrol loaded in the NLC improved the motility, progressive sperm motility, viability, and membrane integrity compared to the control group. The results of this experiment indicated that 40 μM resveratrol, resveratrol loaded in liposome and resveratrol loaded in NLC improved most of the evaluated parameters.