Synthesis of ZnO/Urtica Dioica Nanoparticles by Wet Chemical Procedure and Investigation of Its Antibacterial Activity Against Multiple Drug Resistant (MDR) Staphylococcus aureus Isolates of Diabetic Ulcers

The emergence of new sciences such as nanotechnology and its convergence with engineering, biology and medicine, it is raising hopes for the treatment of infectious diseases. One of the infections that has affected a wide range of people in the community Infection of diabetic wounds, one of the causes of which is the bacterium Staphylococcus aureus. In this study, using nanoscience and combining it with the old herbal medicine plant nettle, which is a perennial herb. And ZnO has great nutritional and medicinal value. Zinc oxide (ZnO) nanoparticles were synthesized by nettle coating. Synthesis of ZnO nanoparticles was performed by wetting the nettle plant by chemical method. Structural evaluation of minister nanoparticles of ZnO synthesized by X-Ray Diffraction (XRD), Transmission Electron Microscopy (TEM), and Fourier Transform Infrared Spectroscopy (FTIR) was performed. The antibacterial effect of this nanoparticle on antibiotic-resistant Staphylococcus aureus isolated from diabetic wounds was investigated. The antibacterial performance of ZnO/Urtica diocia nanoparticles against Staphylococcus aureus Multi Drug Resistant (MDR) isolates was evaluated by qualitative agar well diffusion test at concentrations of 0.032 to 0.256 g/ml of synthesized nanoparticles. The results of this study showed that the size of the synthesized nanoparticles was 100 nm and spindle-shaped and the highest antibacterial properties of these nanoparticles at a concentration of 0.256 g/ml on antibiotic-resistant Staphylococcus aureus isolates with a halo diameter of 20 mm and the lowest an inhibition of growth was observed at a concentration of 0.032 g/ml. According to the findings of this study it seems that ZnO/Urtica dioica nanoparticles had good antibacterial activity against antibiotic-resistant Staphylococcus aureus isolated from diabetic wounds.