The Inhibitory Effect of Nanocomplex of Ganoderma and Silver Fungus Extract on Biofilms of Bacteria that Cause Nosocomial Infections

In general, nosocomial infections are those infections that are limited or diffuse and are caused by pathogenic reactions related to the infectious agent or its toxins in the hospital, provided that at least 48 to 72 hours After admission, the patient should be hospitalized and at the time of admission, the person should not have obvious signs of infection or be in the latent period of the disease. The causes of nosocomial infections are discussed. The bacteria used in this study included standard strains of Staphylococcus aureus (ATCC 14028), Acinetobacter baumannii (ATCC 6538) and Streptococcus pyogenes (19606 ATCC) and Pseudomonas aeruginosa (ATCC 27853). The bacteria used in this study were transferred to their specific media including McConkey agar (Pseudomonas aeruginosa), Parker agar board (Staphylococcus aureus), blood agar (Streptococcus pyogenes) and chocolate agar (Acinetobacter baumannii). Scanning electron microscope was used to measure the dimensions and shape of silver nanoparticles. Infrared spectroscopy was also performed to investigate possible organic compounds that could be involved in the synthesis of nanoparticles. Microplate method was used to investigate the effect of fungal and silver nanocomplexes in inhibiting the biofilm formation of bacteria used in this study. The results showed that there was a significant relationship between biofilm formation in the presence of nano-complex of Ganoderma and silver extracts; Among them, Staphylococcus aureus had a weak biofilm, Acinetobacter baumannii had no biofilm, Pseudomonas aeruginosa had a weak biofilm and Streptococcus pyogenes had a strong biofilm. Fungal and silver nanocomplexes have antibacterial activity in various concentrations and also this complex has a growth inhibitory concentration and also has the ability to inhibit biofilm formation. Due to the good antibacterial activity of this compound, this compound can be used in various medical applications.