Anti-bacterial effects of essential oil of Cardaria draba against bacterial food borne pathogens

Cardaria draba plant is a perennial plant from Brassicaless ordert. Tincture and essence of Cardaria draba was used in traditional medicine to treat various diseases. While its antioxidant effect of Cardaria draba is indicated, its anti-bacterial effects haven’t been checked. In this study, we investigate the antibacterial effect of Cardaria draba essence against some bacterial food borne pathogens in vitro.

This experimental study Having collected the leaves of this plant in spring, we extracted the essence. Also, there are different concentration rates for the essence (like 40, 20, 10, 5, 2.5, 1.25) which have been produced by microdilution Broth method in BHI medium and culturation in Mueller Hinton Agar medium. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) have been determined with two methods of visual monitoring and optical density (OD) for the bacteria Escherichia coli, Salmonella typhymurium, Staphylococcus aureus, Lysteria monocytojenese, and Bacillus cereus with the use of Elisa Reader. The results were statistically analyzed by ANOVA and post hoc Duncan tests.

The results showed that the essence of Cardaria draba with minimum inhibitory concentration of 2.5mg/ml was found effective on staphylococcus aureus and the minimum bactericidal concentration of 40 mg/ml was effective on this bacterium. With different essence concentrations of this plant, no effects of inhibitory or bactericidal on Escherichia coli, Salmonella typhymurium, Lysteria monocytojenese, and Bacillus cereus were found.

Through the results of the study, we found that the essence of Cardaria draba has relatively weak inhibitory effect on Escherichia coli, Salmonella typhymurium, Staphylococcus aureus, Lysteria monocytojenese, and Bacillus cereus. We suggest that there is more room for investigation on the effective particles of the extracts of this plant.