Investigation of the effect of Quercus brantii fruit-hull extract on hen egg-white lysozyme fibrilation and defibrillation

In normal physiological conditions, proteins are mostly spherical and water-soluble. In some cases, proteins are converted into structures called fibrils or amyloid plaques that are insoluble and pathogenic. In this study, the effects of hydroalcoholic extracts of Quercus brantii fruit-hull on the formation of hen egg-white lysozyme fibrils as well as the effect of this extract on the elimination of preformed HEWL fibrils have been investigated. For this purpose, lysozyme was incubated in presence and absence of Q. brantii extract at 60 ˚C for 15 days. The effect of extract was evaluated using CR binding assay, CD spectroscopy, THT and ANS fluorescence testing, and FE-SEM microscope.The results showed that Q. brantii extract in all tested ratios has an inhibitory effect on the formation of amyloid fibrils. Also, the extract has an accelerating effect on the defibrillation of amyloid aggregates and the greatest effect of it on defibrillation is observed in the ratio of 0.5: 1 extract to fibril. From the results obtained in this study, it can be concluded that Quercus brantii fruit-hull contains compounds that can be used as agents in the preparation of drugs for the removal of amyloid plaques in the treatment of amyloid-dependent diseases.