Anti-tumor effects of salvia atropatana Alcoholic extract on ubcutaneous colorectal carcinoma CT26 in Balb/c mouse

Angiogenesis is an essential step in transmitting tumors from dormant to malignant. The use of some angiogenesis inhibitors can be effective in treating cancer. Some changes in cancer cells can be treated with plant extracts. Azerbaijanis belong to the mint family. Salvia species have been used in traditional Iranian medicine and in some other countries against various infections, inflammatory diseases, and cancer. The aim of the present study was to determine the anti-tumor effects of Maryam Goli Azerbaijani extract on colorectal 26 CT subcutaneous cancer in Balb / c mice.

In the current experimental study, 26 CT cells were injected to the right side of 18 Balb / c mice to induce colon carcinoma. When the size of the tumor became (50 ± 350) mm cubic meters, 18 mice were randomly divided into 3 groups, each group consisting of 6 mice: controls and alcoholic extracts were injected with 50 and 100 mg/kg. The group containing the aqueous extracts of the Azerbaijani Sage herb was injected on a daily basis for 14 days. To monitor the therapeutic effects, the parameters of the stopping rate in the growth of the tumor, the relative volume changes, and the doubling of tumor volume were evaluated. After sacrificing the animals at the end the 14th day, tumors were dissected for histological study. Data were analyzed using frequency distribution, mean, and standard deviation tables. The normality of quantitative variables was examined using the Kolmograph-Smirnov method (KS test) and to determine the difference between different groups, I-Whitney test was run in SPSS, v.16. Also, the graphs were plotted using Excele 2007.

Alcoholic extract Salvia atropatana reduced average relative size of tumors in the treatment group dose 50 mg/kg  fom 1468 ± 78 to 900 ± 34 and the average relative size of the tumor at a dose of 100 mg/kg from 1468 ± 78 to 492 ± 78 compared with the control group. Also, alcoholic extract with a dose of 100 mg / kg had the greatest effect. On the 14th day of treatment, there was a significant decrease in the mean tumor volume compared with the control group in the therapeutic group receiving alcohol extract with a dose of 50 mg / kg (P <0.01) and in the treatment group receiving the alcohol extract with a dose of 100 mg/kg (P<0.001).

It seems that the alcoholic extract of Salvia atropatana has an anti-angiogenesis activity and a significant inhibitory effect on tumor growth.