The Effect of Six Weeks of A High-Intensity Interval Training (HIIT) Swimming and Resveratrol Supplements on the Area of Hepatocytes and Polyploid Nuclei in Aged Male Rats Wistar Race

Polyploid nuclei are formed as a result of a defective cell division cycle, which is strongly associated with the aging process and leads to an increase in the area of  hepatocytes, i.e. cell inflammation. The purpose of this study was to evaluate the effect of a period high-intensity interval training (HIIT) swimming with resveratrol (R) supplementation on the area of liver cells and polyploid nuclei in aged rats. In this study, 40 male Wistar aged rats (twenty-month-old, weight of 350-450 g) that the NOVEL test of whom was negative and had no motor disorder, were divided into five groups (control (C), HIIT training (HIT), supplement and HIIT training (HITR), supplement (R), Solvent (S). Rats in the (HIT) and (HITR) groups performed (HIIT) swimming for six weeks (three days a week) the initial load applied (in the first week) was 9% of body weight per rat and 1% was added every week. Also, eight young rats (three-month-old, weight of 150-180 g) was placed in a group(Y) for comparison. At the end of the protocol and after biopsy, 5 healthy tissues were obtained from each group. Variables were stained with Hematoxylin and Eosin (HE) and, ImageJ software was used to calculate the area of hepatocyte and one-way analysis of variance and Tukey’s post hoc test were used to compare the groups. After six weeks of HIIT swimming with and without resveratrol, there was a significant decrease between the mean levels of hepatocytes in the study groups, which was significant in the training group compared to the other groups. However, this change in polyploid nuclei was not significant (p ≤0.05). There was a significant difference between the mean area of hepatocytes in the research groups (P=0.0001) (Table 1). According to Figure 1, Tukey’s test showed that this significant difference was in the mean area of hepatocytes between the control and exercise (P=0.0001), control and youth (P=0.0001), solvent and supplement (P=0.012), solvent and training (P=0.0001), solvent and youth (P=0.0001), supplement and training + supplement (P=0.0001), supplement and training (P=0.002), supplement and youth (P=0.003), training + supplement and training (P=0.0001), and training + supplement and youth groups (P=0.0001). There was no significant difference between the other groups. It seems HIIT swimming exercise with resveratrol supplements can lower liver cell levels.