Investigating the Antimicrobial Effects of Amniotic Membrane, Arnebia Euchroma, Sesame Oil, and Bee Wax on Gram-positive and Gram-negative Bacteria in Second-degree Burn Wounds

Some tissues of the body such as the amniotic membrane and cheap and available medicinal plants such as Arnebia euchroma can be effective in treating burn wounds, reducing its infection, and healing time. The aim of the present study was to investigate the antimicrobial effects of a combination of amniotic membrane, Arnebia Euchroma (AE) extract, sesame oil, and bee wax on the growth of gram-positive and gram-negative bacteria in second-degree burn wounds. After receiving amniotic membrane tissue from pregnant women, this tissue was mixed with sesame oil, bee wax, and Arnebia Euchroma extract and applied to the burn wounds of mice model infected with E. coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Acinetobacter baumannii. This combination was applied in case and control groups, and microbial counting was then performed. According to the findings of the present study, the number of pathogens in wound samples of mice treated with this combination was less than in control mice. The results of this study also showed that the use of a combination of amniotic membrane, AE plant extract, sesame oil, and bee wax had a therapeutic effect on second-degree burn wounds. The use of this compound after further research is recommended for the treatment of burn wounds.