The Effect of Concomitant Use of Grape Seed Extract Coated with Chitosan Nanoparticles and One Period of HIIT on The Rate of Cardiomyocyte Apoptosis of The Myocardial Infarction Model in Rats

Ischemia increases the production of reactive oxygen species and oxidative stress, which leads to cell damage directly by acting on membranes and proteins or indirectly by stimulating apoptotic pathways. One of the plants has antioxidant properties of grape seed extract that can reduce the production of free radicals. The aim of this study was to investigate the effect of concomitant use of grape seed extract coated with chitosan nanoparticles and one period of HIIT on the rate of cardiomyocyte apoptosis of the myocardial infarction model in rats. In this experimental study, 25 male Wistar rats who developed myocardial infarction by subcutaneous injection of isoprenaline(85mg/kg) were selected, Rats in this study were randomly divided into 5 groups:Control, Strok, Strok+Nano-Sup, Strok+AIT and Strok+Nano-Sup+AIT. AIT was performed with 7 intervals of each interval, including 4 minutes with intensity of 80 to 90% Vo2max and 3 minutes with intensity of 65 to 75% Vo2max. Data were analyzed using Shapiro-Wilk statistical tests, one-way analysis of variance and tokey post hoc test at α =0/05. The results showed that Casp9 and Casp3 in the myocardial induction group had a significant increase compared to the healthy control group (P = 0.001). compared with the myocardial infarction group, only the stroke + exercise + grape seed group showed a significant decrease in Casp3 and Casps9 and a significant increase in SOD values (P = 0.001). Therefore, it seems that HIIT and grape extract negatively regulate the inflammatory factor Casp3 and Casps9 of heart tissue can inhibit cardiac cell apoptosis after myocardial infarction and also reduce heart tissue damage.