Evaluation the effect of different concentrations of agar and carbohydrate on the ‎micro-propagation of PHL-C, a dwarf cherry rootstock

This study was conducted to investigate the effect of different concentrations of agar and carbohydrate on micro-propagation of dwarf cherry rootstock (PHL-C) under in-vitro conditions in 2017 year. This experiment was conducted as factorial in a completely randomized design with six replications and three plantlets in each replication. The first factor was agar in three levels (0.5, 0.6, 0.7%) and the second factor was carbohydrate in six levels (sucrose and sugar each in 3 levels of 20, 30, 40 g / l) and evaluated in proliferation, rooting and adaptation steps. The single node was used for propagation The results showed that there was no significant difference between different agar concentrations in terms of proliferation index, rooting percentage, plantlet quality, plantlet fresh weight and survival percentage, but in other measured traits, agar with concentration of 0.7 % had the best results. Except in the traits plantlet height, leaf number and plantlet fresh weight which the sucrose had a more positive effect, in other traits there was no significant difference between sugar and sucrose. Due to the fact that in the essential traits of in vitro propagation of PHL-C, sugar has a better effect, it is suggested to use table sugar or a combination of sugar and sucrose to reduce the cost of producing the culture medium, especially in commercial production.