Anti-proliferative and Apoptosis Induction effect of Iranian Snake Venom (Vipera Raddei Kurdistanica) on Human Breast Cancer (MCF-7) and Normal Breast (MCF-10a) Cell Lines

Breast cancer is the second leading cause of death among women worldwide. Despite recent advances in cancer treatment, this disease remains one of the leading causes of death. Snake venom is a mixture of various molecules such as carbohydrates, nucleosides, amino acids, lipids, proteins and peptides. Previous studies have shown that the venom of some snakes have anti-cancer effects on human cell lines. In the present study, the effect of Vipera raddei kurdistanica venom on breast cell lines was investigated.

The effect of increasing concentrations of snake venom on breast cells viability was investigated using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay and lactate dehydrogenase measurement. Apoptotic cell death was evaluated by fluorescent dye staining and DNA fragmentation assay. Changes in the expression levels of some apoptotic-related genes were investigated by using real time PCR. Data were analyzed by one-way ANOVA followed by Tukey's test. P <0.05 was considered significant.

After 24, 48, 72 and 96 hours treatment, cell viability was significantly decreased in a time and dose dependent manner (P <0.05). The effect of venom was significantly less on normal breast cells than on cancer cells (P <0.05). Apoptotic cell death was significantly increased (P <0.05) in a dose dependent manner. Results of real time PCR confirmed the increase in apoptotic cell death due to venom treatment.

These data indicated that snake venom of Vipera raddei kurdistanica had anti-cancer properties through apoptosis cell death induction specifically in breast cancer cells.