Effects of 12 weeks trans-Resveratrol supplement and endurance training on platelet indices responses to acute exercise in male Wistar rats

Endurance training can reduce the risk of cardiovascular diseases by affecting on platelet indices, moreover it has been shown that resveratrol supplement also has a similar effect of training on platelet function. The aim of this study was to evaluate the effects of 12 weeks of trans-resveratrol supplementation and endurance training on platelet indices in response to exercise.

In this study, 32 male Wistar rats (age, 8 weeks) were randomly divided into 4 groups including control (C), supplement (S), training (T) and training-supplementation (T+S). T+S and T groups performed a 12 weeks of endurance training, 5 days a week, on a motorized treadmill. The training protocol were started for 10 minutes at a speed of 10 m/min in the first week and reached up to 60 minutes at 25 m/min at the end of the training. S and T+S groups received 10 mg trans-resveratrol per kilogram of body weight per day for 5 days a week. Forty-eight hours after the last training session, all groups performed an acute exercise trial at speed of 25 m/min and slope of 10 degrees up to exhaustion. Blood samples were taken immediately after exercise and analyzed by electrical impedance method for platelet indices include platelet count (PLT), mean platelet volume (MPV), platelet distribution width (PDW) and platelet percentage (PLC). Data were analyzed by one-way analysis of variance and Bonferroni post hoc test at the significant level of p<0.05.

The MPV responses to acute exercise were significantly different among the 4 groups (p=0.03). Comparison of the couples showed that the MPV in group S was significantly lower than C (p=0.01) and T (p= 0.01) groups. There was no significant difference between the responses of other indicators to exercise (p>0.05).

Consumption of the trans-resveratrol supplementation for 12 weeks, significantly reduced the mean platelet volume of group S in response to acute exercise. Combining the supplement with training and training had no effect on the response of platelet indices to acute exercise.