Evaluation of Allergic Effects of Aqueous Extract of Fennel on Wheat Grass Germination Factors

Allergic properties of plants are an important factor in the production of natural herbicides. In this study, the microbial antimicrobial effects of aqueous extract of fennel on wheat germ germination factors were investigated.

In this experimental study, a randomized complete block design was performed in 4 treatments with 3 replications. The treatments of this experiment included 4 different concentrations of fennel aqueous extract (15, 10, 5, 5%). Measured parameters included germination percentage, seedling fresh weight, activity of alpha-amylase, catalase and malondialdehyde concentration.

The results showed that the effect of experimental treatments on all measured traits of wheat grass was significant at the level of 1% probability. There was no significant difference with 5% treatment. With increasing fennel concentration, wheatgrass seedling weight also decreased. The highest weight was observed in control (0.11) and the lowest was observed in 15% fennel concentration (0.06). The highest and lowest alpha enzyme activity was observed. Amylase was observed in control treatment (4.53 nmol / min) and 15% fennel extract (1.67 nmol / min), respectively. The highest concentration of malondialdehyde in seedling tissue in 15% extract was 93% (nmol / gram )was observed. Decreased activity of antioxidant enzyme catalase was affected by increasing the concentration of fennel extract. Although the lowest activity of this enzyme was observed at a concentration of 15% (4.33 mg absorption per minute), this treatment differed from the treatment concentration of 10% (4.47 mg absorption per minute) It did not make sense.

According to the obtained results, it can be said that with increasing the concentration of fennel extract, the concentration of malondialdehyde also increased significantly. Aldehyde and decreased alpha-amylase activity.