The first report of mild pepper mild mottle virus in mixed infection with Tomato brown rugose fruit virus from pepper in Isfahan province

Pepper mild mottle virus (PMMoV) is a serious disease in pepper and some other plant species in the Solanaceae family, with significant damage to these crops throughout the temperate to tropical regions (Colson et al. 2010). The causative agent of the Pepper mild mottle disease single-stranded RNA virus belong to the Tobamovirus genous, family Virgaviridae, that induce (Peng et al. 2015). Mixed infection of plant viruses is a common feature in nature which may lead to more severe symptoms than their single infection. During a survey conducted in the fall and summer of 1400, capcicum plant samples showing symptoms of mild mosaic symptoms on leaves and fruit blistering and necrosis, were collected from a number of greenhouses in Isfahan. PMMoV with an another tobamovirus, Tomato brown rugose fruit virus (ToBRFV), may cause more severe symptoms on pepper or tomato plants (Lu et al. 2012). Total RNA was extracted from collected leaves and fruits tissues by using a TRIzol reagent (Sinaclone) and subjected to reverse transcription-polymerase chain reaction (RT-PCR) assay using a PMMoV specific primer pair PMMoVCP / a and PMMoVCP / s (Peng et al. 2015) a 500 bp DNA fragment was amplified from affected pepeer plants. Furthermore, To assess the presence of other viruses in mixed-infection with PPMoV, in plant samples showing severe symptoms, the extracted total RNA was used in an RT-PCR using four primer pairs including: a degenerate primer pair of potiviruses Nib1 / Nib3R (Riechmann et al. 2015);