The effect of ovulin hormone on some hematological, biochemical, hormonal and sperm parameters of male broodstocks of carp (Cyprinus carpio) in the Caspian Sea

Due to use of ovulin hormone in the carp breeding centers and lack enough information about the effect of its different concentrations on this fish broodstocks, evaluation of the effectiveness of ovulin hormone in artificial reproduction of carp is essential. This study was performed on 12 male broodstocks of carp with an average weight of 1307.85±169.57g. Fish in 4 groups (each group with 4 fish) were injected at doses of 0.2, 0.25, 0.3 and 0.35mg/kg body weight of ovulin hormone in one time. The injection method was performed peritoneally and below the pectoral fin. Blood samples were taken from fish in two times, before injection and 10 hours after injection. Based on the results, it was found that injection of different doses of ovulin hormone had no effect on red blood cell counts (RBC), mean corpuscular hemoglobin (MCH) and white blood cell counts (WBC) (P>0.05). However, the amount of hemoglobin, hematocrit and granulocytes in all treatments decreased in the blood of male broodstock after the injection of ovulin hormone and the percentage of monocytes increased (P <0.05). It had no effect on total protein and sodium (P>0.05). However, glucose and calcium levels increased and triglyceride, albumin and potassium levels decreased in the studied doses (P<0.05). The levels of cortisol and sex hormones testosterone, estradiol and progesterone in male broodstocks increased in all doses studied (P<0.05). Also, the highest sperm motility and spermatocrit were observed in 0.2 mg/kg, pH in 0.3 mg/kg, triglyceride and alkaline phosphatase in 0.35mg/kg and calcium, sodium and potassium in 0.25mg/kg (P<0.05). The results of this study showed that doses of 0.25 and 0.3mg/kg body weight had the best effect on blood indices, steroid hormones and sperm indices in male broodstocks of carp.