Changes in BTG1 Gene Expression ‎Level in People with Gastric Cancer ‎and Its Comparison with Healthy ‎People

Gastric cancer (GC) is one of the most common types of cancer and the second leading cause of cancer mortality. Delay in diagnosis and lack of screening are the main causes of high mortality from this disease. Finding an accurate and effective diagnostic biomarker seems to be essential for effective treatment of GC. In this regard, using a gastroscope, we collected tissue samples from patients with GC and healthy individuals. The obtained samples were used to extract their RNA using Trizol solution kit. RNA samples were used for qRT-PCR using specific primers designed for BTG1 and GAPDH genes. QRT-PCR results were analyzed using 2-ΔCt method and various statistical tests using SPSS software. In total, 40 samples of GC and healthy controls were collected and their demographic information was recorded. RNA extraction produced the amount of RNA required for qRT-PCR. The qRT-PCR results showed that BTG1 expression was significantly decreased in GC samples. According to the obtained results, it can be concluded that the reduction of BTG1 expression can act as an accurate biomarker for GC. This gene can also be an indicator of GC pathogenicity. These results could indicate possible diagnostic and therapeutic applications of BTG1 for GC.