Investigation of the effect of salicylic acid on adventitious root and callus secondary metabolites in Echinacea purpurea L. in tissue culture medium
Echinacea purpurea L. is very important in the pharmaceutical industry due to its valuable secondary metabolites. In this experiment, the investigation of salicylic acid in tissue culture medium on callus and adventitious root secondary metabolites of Echinacea was conducted. The best treatment for callus production was MS medium contain 0.5 mg/l BA and 1 mg/l 2,4-D. The best treatment for adventitious root was obtained in 1/2 MS medium contain 0.5 mg/l IBA in dark condition. Salicylic acid at concentrations of 0, 50, 100 and 200 mg/l in liquid MS medium, and then fresh weight, phenol content, flavonoids and antioxidant activities were measured. The highest antioxidant activity was observed in 100 and 200 mg/l of salicylic acid in callus and adventitious root respectively. Salicylic acid promoted phenol and flavonoid content rather than control medium (without hormone). The highest gallic acid content with 2.11 mg/g root fresh weight was recorded in 100 mg/l 3 days after inoculation. Also the best medium for cafeic acid content was observed in 200 mg/g root fresh weight 5 days after inoculation.
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.