Effect of enriched Artemia urmiana with highly unsaturated fatty acid and vitamin C on the reproduction performance of Pterophyllum scalare

Artemia (Artemia urmiana) was added to diets of Angelfish and various factors: fecundity, fertilization rate, hatching rate and larvae survival rate and interval between continuous spawning and total time for 8 spawning (did you run 8 spawning cycles, this is not clear) for Angelfish. five diets were tested (n=3): a 40% crude protein diet (control); control diet + live Artemia, live Artemia; live  artemia +  unsaturated fatty acid live Artemia + unsaturated fatty acid + 1gr vitamin C. Broodstock Angelfish (18 pair) were kept in a 50 L aquarium (28 ± 1°C, pH 7.5-8.3,  hardness <170ppm). Fish were fed 4 times per day between 7:00 and 23:00.
The average rate of fecundity was 378 eggs, 98.9% fertilization, 97.5% hatching for fish fed the live artemia + fatty acid. The artemia + fatty acid + 0.5 g vitamin C resulted in the highest larvae survival (93.3%) which was significantly higher than the control group (P < 0.05). The shortest period for 8 spawning cycles (43 days, 6 day interval) was for the Artemia + fatty acid + 1 g vitamin C. Feeding live food reduced the time for 8 spawning cycles and increasing fecundity, fertilization rate, hatching rate and larvae survival rate. Adding artemia decreases this time between spawns and would be a useful ingredient for continuous spawning operations and is likely to be economically for broodstock operations because of increased efficiency even though the feed cost would be higher.