Detection of Lung Cancer Cell-Derived Exosomes in Microfluidic Platform via Immunofluorescence

 Lung cancer is the most killer cancer in both men and female, as the lung cancer mortality rate is higher than death by the next two breast and prostate cancers. Lung cancer treatment compared to breast cancer is almost unsatisfied, since the most of lung cancer cases are diagnosed at a stage of the disease where metastasis has occurred. The cause of late diagnosis in lung cancer is the similarity of signs and symptoms of cancer with pulmonary symptoms and lack of confidence approaches to identify it at early stage. Nowadays, much attempt is paid to biomarkers such micro RNAs, exosomes, and tetraspanins that can give us information about intracellular events. Microfluidic as science is the study of fluid behavior, and as technology is the construction and use of the specific properties in these dimension which is evolving and entering various fields, from chemical synthesis and biological analysis to optics and even information technology. In this work, we designed and fabricated via UV lithography a microfluidic chip for lung cancer cell derived exosomes capture followed by detection by CD63/CD151-FITC antibodies. The capture exosomes then extracted from microfluidic chip and investigated by Atomic Force Microscopy, AFM, and Dynamic Light Scattering DLS. The average size of exosomes was measured 19-37 nm by DLS and 35-37 nm by AFM that are similar to each other.