Evaluation of the effect of hydroalcoholic extract of nettle plant on the expression of the COX gene in cancer induced mouse ovaries

Ovarian cancer has the highest mortality rate among all genital malignancies. It is the sixth cancer in the western world that has no symptoms at all in the early stages. One of the genes that is highly expressed and inhibited in cancer is the Cox gene. The aim of this study was to evaluate ovarian cancer in early stages, evaluation of Cox gene expression and SGPT and SGOT enzymes, and also investigated the treatment of different concentrations of nettle extract. In this experimental study, hydroalcoholic extract of nettle leaf, stem and root was prepared and doses of 75 and 250 mg/kg body weight were prepared. DMBA was used to induce ovarian cancer in mice. Blood samples were taken after 28 days to measure enzyme levels. Ovarian tissue was then separated, weighed and morphologically compared with other organs. RNA extraction, nanodrape, electrophoresis, cDNA synthesis, and gene expression analysis were performed. The results were evaluated by SPSS software. The results of SGOT enzyme assay showed that R250, S75 and T2 treatments had significant changes. The normal expression level of Cox gene was in the laboratory control (1.63) and the lowest gene expression was related to S75 treatment on day 28 and the highest expression was related to R75 on the same day. Cox gene expression changes and the SGOT enzyme can be used as a marker for early detection of ovarian cancer.