Evaluation of silver nanoparticles effects on bla-per1 gene expression for biofilm formation in isolates of antibiotic-resistant Acientobacter Bumanni by real time PCR method

Acinetobacter bomanni is one of the most common opportunistic pathogen in hospital that is resistant to many antibiotics due to the production of biofilm. : In this experimental study, Acientobacter bumanni were isolates from 100 clinical samples. After identification of Acientobacter bummani strains and determination of antibiotic resistant profiles, biofilm producer isolates were determined using PCR method. The Minimum inhibitory concentration (MIC) of strains against AgNPs was determined. After 24 hours exposure of strains with subMIC concentration of AgNPs, RNA extraction and cDNA synthesis was performed. Finally, evaluation of bla-per1 gene expression was measured using real time PCR method. Results out of 100 clinical isolates, 12 isolates were belonged to Acientobacter bummani and all of strains were resistant to antibiotics except colistin. PCR results show that 12 isolates have bla-per1 gene and they were biofilm positive. Real Time PCR results show that after treatment of isolates with subMIC concentration of AgNPs, all of strains had a significant reduction in bla-per1 gene expression.