Effects of Silymarin and 17β estradiol on gonadosomatic index and histological changes of oocytes in female Three spot Gourami.(Trichogaster trichopterus)

Effects of silymarin and 17β estradiol (E2) on gonadosomatic index and histological changes of oocytes were investigated in female Three spot Gourami. 100 immature female fish with mean weight of (2.5±0.5g) were evaluated in 8 treatments with 2 control groups. The fishes in different groups were treated with silymarin and E2 separately (10,20,30,50 mg/kg). Control one received 20µL methanol and control two was intact. After 20 days experimental fishes ovaries removed and weighted. Histopathological studies of ovary and gonadosomatic indires of fishes of the 8 tretments were compared with those of control groups, fishes. Statistical analysis showed that the results of comparison of amount of GSI for silymarin in first and second treatments was 1.2% and for third and fourth treatments was 1/8% Amount of GSI for (E2) in fifth treatment was 0.7% and sixth treatment was 1.2% and for seventh and eighth treatments it was 1.75% in comparison with control group which was 0.5% these defferences were significant (P<0.05). This result was approved by histopathological finding which showed that fourth and eighth treatment were more effective because begining moving Germinal vesicle on growth and maturation of oocytes in female three Spot Gourami.