Effect of Bunium Persicum Essential Oil,, NaCl and , Bile Salts on viability of Lactobacillus-acidophilus (ATCC4356)

The viability of Lactobacillus acidophilus in the commercial preparation process and its usage as probiotic Bactria is useful. The main purpose of this paper is to study the effect of Bunium persicum essential oil (BEO) stress, NaCl, bile salts, and their combinations on the viability of Lactobacillus acidophilus Bactria. In the following, the effect of BEO stress (0.5 percent), Nacl (2 percent), bile salt (0.15 percent) in comparison with the treatment instance was studied on the Lactobacillus acidophilus population in the refrigerator. Furthermore, the data analysis process was done with the two-way ANOVA and comparing the means by LSD method in SAS software version 9. The conclusions have been out coming from three-time tests with the consideration of P<0.05 in all test steps. After four weeks of 4-Celsius degree temperature conditions, the viability of Bactria and PH decreased, while the amount of degradation in the treated sample was less than the index in the stressed samples. Moreover, the amount of acidity raised in all samples and this growth in stressed samples was more than a treatment sample (P< 0.05). As a conclusion, using BEO with NaCl and bile salt in the densities below of MIC can lead to the viability of Bactria till the end of 14th-day maintenance in the probiotic product.