The effects of aerobic training and eugenol supplementation on GAP43 gene expression and myocardial degradation in rats poisoned with chlorpyrifos

Chlorpyrifos is an organophosphate toxin used to control agricultural pests. These toxins alter the expression of genes and cause cell damage in the body. Due to the high consumption of these compounds in rice fields and citrus orchards and their potential harm, the present study investigated the effect of aerobic exercise and eugenol supplementation on GAP43 gene expression and myocardial damage in rats poisoned with chlorpyrifos. In this study, 36 male Wistar rats were randomly divided into six groups: healthy control, sham, poisoned control, aerobic training poisoning, eugenol poisoning, and toxic + aerobic training+ eugenol. The interventions were performed based on the name of the group and 48 hours after the last intervention, a biopsy was performed. Data were analyzed using one-way analysis of variance and P less than 0.05 was considered as a significant change. The results of the present study showed that poisoning significantly reduced the expression of this gene and the destruction of collagen in heart tissue. Collagen degradation was trained in poisoned rats and consumed less eugenol, while GAP43 gene expression in poisoned rats did not change significantly due to exercise and supplementation alone. The interaction of exercise and supplementation increased the expression of the GAP43 gene in the hearts of poisoned rats and decreased the collagen degradation of their hearts. In general, the simultaneous use of exercise and supplementation caused more beneficial effects on the heart tissue of poisoned rats in the variables of the present study