Hemophilia A disease: Identification and prediction of new microRNA

Various mutations in factor VIII locus are led to an X-linked bleeding disorder in hemophilia A. One of the leading causes of inefficient remedy for hemophilia A disease is the lack of specific and sensitive procedure for punctual diagnosis of the disease. miRNAs indicate that they have a great potential as diagnostic and prognostic biomarkers due to their stability in body fluids and their capability to identification. Furthermore, a relationship between alteration in expression levels of miRNAs and onset and progression of the hemophilia A disease has been reported.Prediction of new miRNAs and nomination as regulators of FVIII gene has been considered as the objective of this study. The ability to express new miRNAs in FVIII locus was studied via reliable bioinformatic databases such as SSCprofiler, RNAfold, miREval, FOMmiR, MaturBayes, miRFIND, UCSC genome browser, Deep Sequencing, and miRBase. Output data of previously mentioned databases were analyzed, and one stem-loop structure was qualified, and the region is predicted to express new miRNA. The presented stem-loop structure can be used as biomarker in diagnosis of the disease and regulation of factor VIII gene after subsequent experimental verification.