Evaluating the oxidative potential of FeSo4 and H2O2 on neutralization of etidium bromide; a material with cancer inducing characteristic for genomic DNA colouring

In biology considering identification of sequencing of macromolecules, different techniques such as Electrophoresis agarose gel is used after separation from different cells. Ethidium Bromide is one the materials for coloring these gels throughout the world due to it’s low price comparing to other materials. In last years, extensive use of his material in biology and medicine labs and due to it’s solubility and persistency in water, natural ecosystems contamination has became an important issue. Entering the human body can lead to enhanced cancer incidence by disturbance of blood circulatory system, activation of different tissues and modulation of translation and transcription of DNA process. Several physical and chemical methods has been suggested for neutralization of Ethidium Bromide inducing BDD electrode, TiO2, activated carbon adsorption and etc. which nearly all of these methods have not been used extensively by investigators due to high price and complex procedures. Fenton’s oxidation is one of materials for neutralization of unfavorable chemical compounds and this material has not been studied for neutralization of Ethidium Bromide. Due to high utilization of Ethidium Bromide in labs, the aim of this study was to investigate the potential effect of different levels of two chemical materials Feso4 and H2O2 for neutralization of Ethidium Bromide. 6 treatments including different levels of Feso4 and H2O2 were investigated. The treatment containing 200 Mg / liter of Feso4 along with 300 Mg / liter H2O2 had the most potential for neutralization of Ethidium Bromide comparing to other treatments.